| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154846 |
| Chromosome: | chromosome_10 |
| Location: | 4412214 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g451600 | sense | intron|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGACAATGAGAGCTGGTTGCGCACACGTG |
| Internal bar code: | GACAGACTCAACGAAACGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.631 |
| LEAP-Seq n confirming: | 1350 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGCCTTGTATGCACCAT |
| Suggested primer 2: | GATGGGACGTATTCATTGGG |