| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.155749 |
| Chromosome: | chromosome 16 |
| Location: | 1928647 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656150 | (1 of 1) PTHR23504:SF1 - PROTEIN T25D3.4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTATTATATAGTTTCTGTGTGCTACTCT |
| Internal bar code: | TTTGCGCATGCACAAGCGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 99.8095 |
| LEAP-Seq n confirming: | 3144 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACCTTAGCAGCAAAAT |
| Suggested primer 2: | CACGAAGTTTGTCCAGCTCA |