| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157387 |
| Chromosome: | chromosome 5 |
| Location: | 2817261 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237400 | DAE1 | (1 of 1) 5.1.1.7 - Diaminopimelate epimerase; Diaminopimelate epimerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATAGCGCGCCCCGAGACCCGACTTCGCT |
| Internal bar code: | ACGCGTCAGGAAGCGTTACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1035 |
| LEAP-Seq percent confirming: | 96.7296 |
| LEAP-Seq n confirming: | 769 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTCGGTCATGGAGACAT |
| Suggested primer 2: | GTCTGAGCGTTGTGTGCTGT |