| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.157451 |
| Chromosome: | chromosome 16 |
| Location: | 1928677 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656150 | (1 of 1) PTHR23504:SF1 - PROTEIN T25D3.4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACGACCGGCTTGGCCTGACGTTATAAG |
| Internal bar code: | GCATAGACCGCTTGTCGCTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 99.87 |
| LEAP-Seq n confirming: | 2304 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAAGTTTGTCCAGCTCA |
| Suggested primer 2: | ACGCACCTTAGCAGCAAAAT |