| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157501 |
| Chromosome: | chromosome 5 |
| Location: | 2814075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237400 | DAE1 | (1 of 1) 5.1.1.7 - Diaminopimelate epimerase; Diaminopimelate epimerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATGAGCTCACAAATGCTGAACCGGCAG |
| Internal bar code: | CAAGCAGCCAGATCGTTTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.8092 |
| LEAP-Seq n confirming: | 5755 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACCTGTCAGATACTCA |
| Suggested primer 2: | GGCAGAGCGAAGATAACCTG |