| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157865 |
| Chromosome: | chromosome 9 |
| Location: | 1354714 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398650 | (1 of 1) PTHR21737:SF4 - CACTIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGCACGGCGCAGCCGCACACCTGTC |
| Internal bar code: | AGCAAGACAAGGATTTTTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1103 |
| LEAP-Seq percent confirming: | 98.2927 |
| LEAP-Seq n confirming: | 2418 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGACGAGGGAGAAGAGG |
| Suggested primer 2: | GCTTTTGTTCAGGGAAGCTG |