Mutant strain: LMJ.RY0402.157865

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.157865_1 Cre09.g398650 (1 of 1) PTHR21737:SF4 - CACTIN 3'UTR 73
LMJ.RY0402.157865_2 Cre10.g421400 (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase intron 73
LMJ.RY0402.157865_3 Cre10.g458350 SRR16 (1 of 1) PTHR22803//PTHR22803:SF55 - MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED // C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 162; Scavenger receptor cysteine rich (SRCR) protein intron 73
LMJ.RY0402.157865_4 Cre15.g636050 CPLD31 Conserved in the Plant Lineage and Diatoms; (1 of 2) PTHR35514//PTHR35514:SF1 - FAMILY NOT NAMED // THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC intron 73
LMJ.RY0402.157865_5 Cre15.g636050 CPLD31 Conserved in the Plant Lineage and Diatoms; (1 of 2) PTHR35514//PTHR35514:SF1 - FAMILY NOT NAMED // THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC CDS 58
LMJ.RY0402.157865_6 Cre16.g657300 CPI1 Cyclopropyl isomerase-like protein; (1 of 1) 5.5.1.9 - Cycloeucalenol cycloisomerase / Cycloeucalenol--obtusifoliol isomerase 3'UTR 73

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Paromomycin, screen 3 of 6 (~20.0% improved growth)
Paromomycin, screen 4 of 6 (~20.0% improved growth)
Paromomycin, screen 5 of 6 (~20.0% improved growth)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.