| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157865 |
| Chromosome: | chromosome 10 |
| Location: | 545498 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421400 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGATAATACCTATGGGGGGGCCGAGGCGT |
| Internal bar code: | TTAGGTGGATACGCGTGAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 93.0249 |
| LEAP-Seq n confirming: | 5228 |
| LEAP-Seq n nonconfirming: | 392 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTATTTCGCTCTCCCT |
| Suggested primer 2: | GCTGCTGCTTCCTCTGTTTT |