Insertion junction: LMJ.RY0402.157865_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157865
Chromosome:chromosome 15
Location:387856
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g636050 CPLD31 Conserved in the Plant Lineage and Diatoms; (1 of 2) PTHR35514//PTHR35514:SF1 - FAMILY NOT NAMED // THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTTACAGCATTCGTGCTTAAGGCTATAG
Internal bar code:TGCAACTAGGGAAAACCAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:919
LEAP-Seq percent confirming:99.6739
LEAP-Seq n confirming:7948
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGCTGGAGAGCTAGCGA
Suggested primer 2:TCTGGTCCCATTCACACGTA