| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158112 |
| Chromosome: | chromosome 6 |
| Location: | 5346924 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283500 | POB3 | Proteome of basal body 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCACACCCCACTCGCAGCTACCTACTAC |
| Internal bar code: | GGCGGGTAACCCAGGGGGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 58.4375 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 133 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGGTTGAGCAGTTTGAG |
| Suggested primer 2: | ACCGGGTTTTTGGTGTAATG |