Insertion junction: LMJ.RY0402.158291_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.158291
Chromosome:chromosome_12
Location:8335128
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g549550 RSP19,TUB2 Beta tubulin 2 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CGATCTGGTTGCCGCACTGGCCACCCTGTG
Internal bar code:ATATGTGGCCGTGGAGTCCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:84
LEAP-Seq percent confirming:99.8182
LEAP-Seq n confirming:549
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TAACTTATGGAGCGCCTGCT
Suggested primer 2:GAGAGTGGCAGACCTGGAAG