| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158543 |
| Chromosome: | chromosome 10 |
| Location: | 2814714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439350 | (1 of 3) PTHR17130:SF24 - GAN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGCTTGGCCAGAGGGCTGTCGTACCTG |
| Internal bar code: | GGGCGGTAAAGCAATGGCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5 |
| LEAP-Seq percent confirming: | 1.62482 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 666 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGACGTGGCCCAGTTTAG |
| Suggested primer 2: | CCGGATAACTGAAACCCAGA |