Insertion junction: LMJ.RY0402.158560_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158560
Chromosome:chromosome 12
Location:9211229
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g541800 (1 of 2) 3.4.21.26 - Prolyl oligopeptidase / Prolyl endopeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTTCAACAGGCGGACGGGCAAAACGAT
Internal bar code:AACCGAACCGATGGTTTTCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:504
LEAP-Seq percent confirming:76.2963
LEAP-Seq n confirming:103
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGAAGCTGGTGAATGAGA
Suggested primer 2:GAGGAGGACAAGCTGACTGG