| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158662 |
| Chromosome: | chromosome 12 |
| Location: | 5111837 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526883 | (1 of 18) PF12906 - RING-variant domain (RINGv) | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACTGCAGCTGTTGACGCTGCAGCTGC |
| Internal bar code: | GGGGCTCCTGCCTAGCCCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 99.6927 |
| LEAP-Seq n confirming: | 5191 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGTGTGTTTGACCTAGT |
| Suggested primer 2: | GAGAGCAGTGGCTGTGTCAG |