| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159462 |
| Chromosome: | chromosome 12 |
| Location: | 5261648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528400 | (1 of 1) PTHR22938:SF0 - ZINC FINGER PROTEIN 598 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCTATCGGCTGTACCAAAGATTGCCTG |
| Internal bar code: | GCGCCGCCTGCAGCCCGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.8529 |
| LEAP-Seq n confirming: | 679 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGCGAAGAGTAGACTG |
| Suggested primer 2: | CAAGAACTACGGCGTGAACA |