| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159736 |
| Chromosome: | chromosome 7 |
| Location: | 4817223 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345900 | DCL3 | Dicer-like protein; (1 of 1) PTHR11207//PTHR11207:SF0//PTHR14950 - RIBONUCLEASE III // RIBONUCLEASE 3 // HELICASE-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGGTGAGGGTTGGGGGGAAACAGAGG |
| Internal bar code: | AGGCGAAGGGGGCAAAGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 99.187 |
| LEAP-Seq n confirming: | 244 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAACCCAAGGTGCGTGTA |
| Suggested primer 2: | AAAAACCGTTGCAATAACCG |