| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159738 |
| Chromosome: | chromosome 11 |
| Location: | 3554096 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481550 | (1 of 1) K03136 - transcription initiation factor TFIIE subunit alpha (TFIIE1, GTF2E1, TFA1, tfe) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGATTTGGCCGGGTCCCATGCAGCGCA |
| Internal bar code: | GCCGAAGGTCACGGCTAACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 99.6904 |
| LEAP-Seq n confirming: | 966 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGAGAAAGCGCAACACA |
| Suggested primer 2: | AGCAAGGCTCTGTACCTGGA |