Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.160053 |
Chromosome: | chromosome_13 |
Location: | 2254709 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g578750 | TBA1 | PsbA translation factor | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCTCGGACCGCCTGGAGACAAGACAGCGG |
Internal bar code: | GTTAGCCTAGTTTCCCTTTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 737 |
LEAP-Seq percent confirming: | 99.0385 |
LEAP-Seq n confirming: | 103 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGAAGCAACGACGGATAC |
Suggested primer 2: | CAGAAGAAGAGGAACCCGTG |