| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160053 |
| Chromosome: | chromosome 13 |
| Location: | 2254709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578750 | TBA1 | (1 of 1) K00207 - dihydropyrimidine dehydrogenase (NADP+) (DPYD); Translation factor for chloroplast psbA mRNA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCGGACCGCCTGGAGACAAGACAGCGG |
| Internal bar code: | GTTAGCCTAGTTTCCCTTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 99.0385 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAAGCAACGACGGATAC |
| Suggested primer 2: | CAGAAGAAGAGGAACCCGTG |