Insertion junction: LMJ.RY0402.160053_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.160053
Chromosome:chromosome_13
Location:2254709
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g578750 TBA1 PsbA translation factor antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TCCTCGGACCGCCTGGAGACAAGACAGCGG
Internal bar code:GTTAGCCTAGTTTCCCTTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:737
LEAP-Seq percent confirming:99.0385
LEAP-Seq n confirming:103
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGAAGCAACGACGGATAC
Suggested primer 2:CAGAAGAAGAGGAACCCGTG