| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160557 |
| Chromosome: | chromosome 12 |
| Location: | 3628275 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514050 | GSF1 | (1 of 1) 1.4.7.1 - Glutamate synthase (ferredoxin) / Ferredoxin-dependent glutamate synthase; Ferredoxin-dependent glutamate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCGAGTACGGCTAGCACGCCCCGTTG |
| Internal bar code: | GGCCGATGCTTGTTCCCCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 99.8836 |
| LEAP-Seq n confirming: | 858 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGCGAGTGGACTTACG |
| Suggested primer 2: | CGACCATCATTCACACCAAG |