Insertion junction: LMJ.RY0402.160721_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.160721
Chromosome:chromosome_13
Location:2136010
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g577950 VPS60 Subunit of the ESCRT-III complex antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGAGGAGCAACCGCTTGTTGATGAGCGGG
Internal bar code:TACCCGGCTATCTTCGGTGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:522
LEAP-Seq percent confirming:99.6441
LEAP-Seq n confirming:840
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTACTCTCGTCGCACCCTCT
Suggested primer 2:ACACACGCACACACACACAC