| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160975 |
| Chromosome: | chromosome 2 |
| Location: | 3243117 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095077 | (1 of 1) PF00571//PF04134 - CBS domain (CBS) // Protein of unknown function, DUF393 (DUF393) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGGCGTAACCCCATGCGCGCAGGCGTC |
| Internal bar code: | CCGCCGGACTGTTAATCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 99.4286 |
| LEAP-Seq n confirming: | 348 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGCACTGTGTCTGTTG |
| Suggested primer 2: | GGATTGAGCCTCGTCCATAA |