| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161080 |
| Chromosome: | chromosome_9 |
| Location: | 566838 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g403800 | DYHB,ROC30,ROC54,ODA4 | Flagellar outer arm dynein heavy chain beta, involved in circadi | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCACGCAGTTGTGTGGCTTGTACGTTTCC |
| Internal bar code: | AGTTCCGACGGCGAACTCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1304 |
| LEAP-Seq percent confirming: | 99.4443 |
| LEAP-Seq n confirming: | 3400 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGACAGCAGCTTGGTA |
| Suggested primer 2: | CATGAACAAGATGGTGCTGG |