| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.161161 |
| Chromosome: | chromosome_7 |
| Location: | 4082588 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g340450 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTCACAAACACAGCTGCCACGGTGTAACT |
| Internal bar code: | TCAAATCAATCGCGCTACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 99.403 |
| LEAP-Seq n confirming: | 5495 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCGTGGTATGCGGTAA |
| Suggested primer 2: | AGCTTGGACAGTGTGTGACG |