| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161279 |
| Chromosome: | chromosome_16 |
| Location: | 1279084 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g651400 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACACTCTGCATAGACCACTGCTGACACTCT |
| Internal bar code: | AGGCGCCCAGTGCTTGCTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 99.8239 |
| LEAP-Seq n confirming: | 567 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTATAACCCATCCGCCAC |
| Suggested primer 2: | ACTCGACCAGTTGTGTGCTG |