| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161450 |
| Chromosome: | chromosome 12 |
| Location: | 8144557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551200 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAGGCAACATAATATTCCGCGCCCGGT |
| Internal bar code: | TTTGTGATCATGGACATGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 99.5444 |
| LEAP-Seq n confirming: | 2185 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCCTGTAGAGGTCGCA |
| Suggested primer 2: | ACATTCGACACTGTGACCCA |