Insertion junction: LMJ.RY0402.161450_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161450
Chromosome:chromosome 12
Location:8144801
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g551200 (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGGCGGGCAGGGAGGTGAGGGCAGGGGG
Internal bar code:GAACGGCTGGGCGTTTCAACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:52
LEAP-Seq percent confirming:96.4871
LEAP-Seq n confirming:824
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACATTCGACACTGTGACCCA
Suggested primer 2:TAGGACAAAAACTGGACGGG