| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.161662 |
| Chromosome: | chromosome 10 |
| Location: | 2813690 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439350 | (1 of 3) PTHR17130:SF24 - GAN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCCCCTCACCTCGCGTCTCCCTCACTC |
| Internal bar code: | CACTATTCGTGCCTGCCTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 99.6007 |
| LEAP-Seq n confirming: | 3991 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTCAGCGTATGAGCCTT |
| Suggested primer 2: | CCCTGCAGTGGAATCAATTT |