Insertion junction: LMJ.RY0402.161761_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.161761
Chromosome:chromosome 4
Location:1921179
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g218150 PPH1,PPP18 Thylakoid phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTACAGAGAGGTGCAGCGAAGTGGACAGC
Internal bar code:CACGGGAAGGCTTGCGCCCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:530
LEAP-Seq percent confirming:99.4008
LEAP-Seq n confirming:1493
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGCGTGTTTGTGTGGTT
Suggested primer 2:AGGTCGGAACCACAAGAATG