| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.162028 |
| Chromosome: | chromosome 14 |
| Location: | 1846329 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620300 | ASB1,ANS2 | Anthranilate synthase, beta subunit; (1 of 1) K01658 - anthranilate synthase component II (trpG) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCCCTGGATGTGCGGGTACTTCTTGTG |
| Internal bar code: | GGGGGCCAACGCTCCTGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 99.768 |
| LEAP-Seq n confirming: | 430 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTATAAACCCCTTGCACGC |
| Suggested primer 2: | CTGGTGGTCCAGATGGAGAT |