| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.162029 |
| Chromosome: | chromosome_7 |
| Location: | 4079336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g340450 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCTACAGCGTGCAGGCGCTGTTCCTCAC |
| Internal bar code: | GCTGGCTCGGGTGTGCATCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 82.5263 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTACAACTCCAGCAGCTT |
| Suggested primer 2: | CAATGGTGATGATGGTCTGG |