| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.162029 |
| Chromosome: | chromosome 7 |
| Location: | 4079336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340450 | PKHD1 | (1 of 1) PTHR11915:SF310 - PICKLED EGGS, ISOFORM A; Similar to Fibrocystin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCCACCGTCAGCGCGAACGACTGCTGG |
| Internal bar code: | TTTTCATCCGAGAGGATAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.4669 |
| LEAP-Seq n confirming: | 933 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTACAACTCCAGCAGCTT |
| Suggested primer 2: | CAATGGTGATGATGGTCTGG |