Insertion junction: LMJ.RY0402.162329_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.162329
Chromosome:chromosome 12
Location:9168531
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g542250 TUB1,RSP19 (1 of 2) K07375 - tubulin beta (TUBB); beta-1 tubulin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAACACGAAGTTGTCCGGGCGGAAAATC
Internal bar code:GTAAGGGCCGCGGTGGGCATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:703
LEAP-Seq percent confirming:99.1731
LEAP-Seq n confirming:1799
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACTTGACGTTGTTGGGG
Suggested primer 2:GAGATCGTCCACATCCAGGT