| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163797 |
| Chromosome: | scaffold 24 |
| Location: | 70542 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755647 | (1 of 1) K14404 - cleavage and polyadenylation specificity factor subunit 4 (CPSF4, YTH1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGTGGTGGCGGCGTTGATTTGGTCTGG |
| Internal bar code: | GTGCGTAGGCCGGCCTATGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 85.2095 |
| LEAP-Seq n confirming: | 5329 |
| LEAP-Seq n nonconfirming: | 925 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGTAGCAGGCAAGGTC |
| Suggested primer 2: | TTGTTTCCTTGTGTGTCCCA |