| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164261 |
| Chromosome: | chromosome 1 |
| Location: | 7498596 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCTCCACGCACTTGCAGCTGTTCGACA |
| Internal bar code: | GGTTCTGGGTCACCAGAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 99.7118 |
| LEAP-Seq n confirming: | 15221 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAGTCGTGCTCATCACTC |
| Suggested primer 2: | AAAGAGCTTGAAGAAGGGGC |