| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164599 |
| Chromosome: | chromosome 11 |
| Location: | 1469733 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467738 | (1 of 8) K13348 - protein Mpv17 (MPV17) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTACCATGCCACGCCCGTCACCCACCGT |
| Internal bar code: | GAGGCATACCCGGGTCCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 98.339 |
| LEAP-Seq n confirming: | 2013 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACTCTCTTCCCGACAGC |
| Suggested primer 2: | AGGTTCTGCCAAATACGGTG |