| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165360 |
| Chromosome: | chromosome 1 |
| Location: | 1948154 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010450 | (1 of 19) PF00397 - WW domain (WW) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGACATGCGGTGGAGCTCGCGAGGCAGG |
| Internal bar code: | TTTCATTCAGGAGGTATTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 98.9758 |
| LEAP-Seq n confirming: | 7248 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGCGTCTTGCAAATCA |
| Suggested primer 2: | GACCGACTGCAGTGTTCAGA |