Insertion junction: LMJ.RY0402.165479_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.165479
Chromosome:chromosome 3
Location:5885822
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g189800 CYN38 Cyclophilin 38; (1 of 1) PTHR11071//PTHR11071:SF236 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP38, CHLOROPLASTIC CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCCCGTAAATCCGCGGGGAGAGGTTGA
Internal bar code:CAAGCTTGTAGTATTATAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1279
LEAP-Seq percent confirming:97.3429
LEAP-Seq n confirming:4726
LEAP-Seq n nonconfirming:129
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGGCCACCAAAGGGTAG
Suggested primer 2:GCGCAATGAGTTTGAGAACA