| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.165558 |
| Chromosome: | chromosome 12 |
| Location: | 1729507 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512700 | (1 of 1) PTHR12716:SF8 - TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCGAACTCTCGGACTGCGGCCTCTCA |
| Internal bar code: | TAGGGACAAAGGACATCTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 99.0506 |
| LEAP-Seq n confirming: | 4382 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGTGACTGAATCTCCG |
| Suggested primer 2: | GAGAACTCTGCAGACCCCAG |