Insertion junction: LMJ.RY0402.165594_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.165594
Chromosome:chromosome 3
Location:1431196
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGTACATGTGTTGTGACCCGACTCGGTG
Internal bar code:CCCTGGCATCGAACCGCACAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1047
LEAP-Seq percent confirming:99.74
LEAP-Seq n confirming:3836
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AACACATGAGGAGCTGGGAC
Suggested primer 2:TCTTGCTAGGTGCGACATTG