| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.166311 |
| Chromosome: | chromosome 1 |
| Location: | 2564177 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCCGCTGGCACCTTGCATTTAGGGATG |
| Internal bar code: | GGGATCCGCCGCCCGGCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 91.1217 |
| LEAP-Seq n confirming: | 1909 |
| LEAP-Seq n nonconfirming: | 186 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTTGTCAATCATGGCAC |
| Suggested primer 2: | ACCTTGAGCACGAAGCTGAT |