Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.166500 |
Chromosome: | chromosome_3 |
Location: | 3477426 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g167550 | POC3,CEP290 | Centriole proteome protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCCTTCAGCAGCGCCACCAGCACCTTGTC |
Internal bar code: | GGTCCGATTACTTCACATCAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 509 |
LEAP-Seq percent confirming: | 99.5471 |
LEAP-Seq n confirming: | 12530 |
LEAP-Seq n nonconfirming: | 57 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCCGGTTTTTGTACTCGT |
Suggested primer 2: | ATCGCTGAAGGTCTGCATCT |