| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.166909 |
| Chromosome: | chromosome 12 |
| Location: | 5861371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533750 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACTAGTATGCGGAGCCAGGGGCTTTGC |
| Internal bar code: | GAGGGGATTTACTTTGGTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1186 |
| LEAP-Seq percent confirming: | 99.1917 |
| LEAP-Seq n confirming: | 2209 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCACTCATTCACGCTCTG |
| Suggested primer 2: | CAGTCTGACCCTGAAGAGCC |