| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.167364 |
| Chromosome: | chromosome_5 |
| Location: | 1346177 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g244701 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCGCGGTAGCCGCGCGCGTCATAGCACA |
| Internal bar code: | CTATCTGAGGCCTCAGCAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 413 |
| LEAP-Seq percent confirming: | 99.7228 |
| LEAP-Seq n confirming: | 2518 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGGATGAGATGGTTTGT |
| Suggested primer 2: | GTAAGGGACGGAGCCATACA |