| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.167521 |
| Chromosome: | chromosome 5 |
| Location: | 2501072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235700 | (1 of 2) K12382 - saposin (PSAP, SGP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGAATTTTGTTGCTGGGTGTATAAAAT |
| Internal bar code: | GCAGTGTCTGGTGAGCTCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 99.4805 |
| LEAP-Seq n confirming: | 4787 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAATCCTATTCCGCCACG |
| Suggested primer 2: | TTCAGAGCGACACACGAAAC |