| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.168204 |
| Chromosome: | chromosome 4 |
| Location: | 2952909 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224750 | VAM1,VAMP1,VAMP71 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III); (1 of 3) PTHR21136//PTHR21136:SF4 - SNARE PROTEINS // N-SYNAPTOBREVIN, ISOFORM J | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACCCCGCTGCCGTCGTTGGTTTCTGTC |
| Internal bar code: | GGGGACCTATCCGGCGGATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACAACCCTCTATCCCA |
| Suggested primer 2: | ATGATGCCCTTCACCTCATC |