| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.168300 |
| Chromosome: | chromosome 12 |
| Location: | 1454724 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487700 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; Amidated peptide precursor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACAGCAGCACCACGTCGGGCGTCTTGCC |
| Internal bar code: | AAGGCACACGCATGGTGTCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 97.7528 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGTACCGTAGTGCCGT |
| Suggested primer 2: | ATGGCATAATCTCGTTTGGC |