| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168310 |
| Chromosome: | chromosome 12 |
| Location: | 4274302 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519500 | CAX1,VCX1 | (1 of 3) K07300 - Ca2+:H+ antiporter (chaA, CAX); CAX family cation antiporter, membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGAACACATGAAACGACACGCTCAGAG |
| Internal bar code: | CCGTGGTGACAATTTAGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 42 |
| LEAP-Seq percent confirming: | 97.9545 |
| LEAP-Seq n confirming: | 1293 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGATCGAATGTGGTTTTG |
| Suggested primer 2: | TGGCGTGAGTATGACCATGT |