Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.168619 |
Chromosome: | chromosome_1 |
Location: | 124715 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g000950 | MFT13 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACCTAATAACGCCCACGTCTGCGGCCTTC |
Internal bar code: | GCTCTGAGTAATCGCATGCTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 41 |
LEAP-Seq percent confirming: | 98.0419 |
LEAP-Seq n confirming: | 5658 |
LEAP-Seq n nonconfirming: | 113 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTGAACAACTTGGGCTAC |
Suggested primer 2: | CCAGCAGCACACAGAACACT |