Insertion junction: LMJ.RY0402.168619_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.168619
Chromosome:chromosome_3
Location:8039295
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTGCCACGTTGCTTTGACATGATACAACCG
Internal bar code:TACTTCTCCGTACTCAGACAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:233
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGAGTCCCGAGAAACAAG
Suggested primer 2:CTCGTACCAGCAGCCAGTG