| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.168619 |
| Chromosome: | chromosome_3 |
| Location: | 8039296 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g201400 | PPP16,PP2C6 | Phosphoprotein phosphatase 2C | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGATGTTCCCAGCAGGCCTCAAGCGCCGA |
| Internal bar code: | GCTCGGCGAATGTGGCACGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 85.1852 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAGTCCCGAGAAACAAG |
| Suggested primer 2: | CTCGTACCAGCAGCCAGTG |